Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human T47D whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 19/KRT19 antigen affinity purified polyclonal antibody (BA4154) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for Cytokeratin 19/KRT19 at approximately 44 kDa. The expected band size for Cytokeratin 19/KRT19 is at 44 kDa.
Figure 2. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154) .
Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 19/KRT19 Antibody (BA4154) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154) .
Cytokeratin 19/KRT19 was detected in frozen section of human placenta tissue. The tissue section was incubated with rabbit anti-Cytokeratin 19/KRT19 Antibody (BA4154) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. ICC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154).
Cytokeratin 19/KRT19 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-Cytokeratin 19/KRT19 Antibody (BA4154) at a dilution of 1:100 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. Western blot analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human T47D whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 19/KRT19 antigen affinity purified polyclonal antibody (BA4154) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for Cytokeratin 19/KRT19 at approximately 44 kDa. The expected band size for Cytokeratin 19/KRT19 is at 44 kDa.
Figure 2. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154) .
Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 19/KRT19 Antibody (BA4154) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154) .
Cytokeratin 19/KRT19 was detected in frozen section of human placenta tissue. The tissue section was incubated with rabbit anti-Cytokeratin 19/KRT19 Antibody (BA4154) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. ICC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (BA4154).
Cytokeratin 19/KRT19 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-Cytokeratin 19/KRT19 Antibody (BA4154) at a dilution of 1:100 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.