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Anti-Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF

M02101-6

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.

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  • Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19(Cat# M02101-6).

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  • Figure 3. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)

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  • Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)

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  • Figure 5. Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)

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  • Figure 6. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)

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  • Figure 7. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.

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  • Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.

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  • Figure 9. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.

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  • Figure 10. Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.

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  • Figure 11. Anti-KRT19 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT19 .

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产品名称
Anti-Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Cell and organelle markers; CK 19; CK19; Cytokeratin 19; Cytoskeleton Marker; K19; K1CS; keratin 19; KRT19
产品类型
Monoclonal
检验物种
human, monkey, rat, dog
应用范围
IF, IHC, WB
基因名称
KRT19
克隆号
OTI3F8
宿主
Mouse
抗体亚型
IgG1
免疫原
Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
计算分子量
44 kDa
实际分子量
44 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
0.15 mg/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Uniprot ID
P08727  
文献引用格式
Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8) (Boster Biological Technology, Wuhan, China. Catalog#M02101-6)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
keratin 19
蛋白名全称
Keratin, type I cytoskeletal 19
推荐稀释比
Western blot (WB):1:1000
Immunohistochemistry (IHC):1:50~150
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100

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    Anti-Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8)

    筛选器: All WB IHC ICC/IF

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