Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |
Figure 1. Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF.
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF.
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 2. IF analysis of STAT3 using anti-STAT3 antibody (BM4169).
STAT3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-STAT3 Antibody (BM4169) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).