Anti-STAT3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0540

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- STAT3 antibody (PB0540). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: ANA-1 whole cell lysates,
Lane 2: COS-7 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
Use rabbit anti- STAT3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for STAT3 at approximately 79KD 86KD ，The expected band size for STAT3 is at 89KD.
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Figure 2. IHC analysis using anti- STAT3 antibody (PB0540). detected in paraffin-embedded section of Human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-STAT3 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

Acute phase response factor; APRF; HIES; STAT3

产品类型

Polyclonal

检验物种

human, monkey, mouse, rat

应用范围

WB,IHC

基因名称

STAT3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human STAT3 identical to the related mouse and rat sequences.

计算分子量

88 kDa

实际分子量

79 kDa86 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21. It contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Uniprot ID

P40763

文献引用格式

STAT3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0540)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

signal transducer and activator of transcription 3 (acute-phase response factor)

蛋白名全称

Signal transducer and activator of transcription 3

实验方案>

实验步骤

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