规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
leucine rich repeat containing G protein-coupled receptor 5; FEX; GPR49; GPR67; GRP49; HG38; LGR5
免疫原
A synthesized peptide derived from human LGR5/GPR49
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Leucine?rich repeat G?protein?coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven?transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G?protein?independent mediators of the potentiating effect of R?Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R?Spondins, Frizzled Wnt receptors and LRP Wnt co?receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal LGR5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).
文献引用格式
LGR5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4244)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Leucine-rich repeat-containing G-protein coupled receptor 5
蛋白名全称
Leucine-rich repeat-containing G-protein coupled receptor 5
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| ImmunoPrecipitation (IP): | 1:20 |
| Flow Cytometry (FCM): | 1:20 |
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