Anti-ATG5 Antibody (Clone#GCD-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4603

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ATG5 antibody (BM4603). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG5 antigen affinity purified monoclonal antibody (BM4603) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG5 at approximately 55 kDa. The expected band size for ATG5 is at 32 kDa.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ATG5 Antibody.
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Immunofluorescent analysis of Raji cells, using ATG5 Antibody.
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产品简介>

产品名称

Anti-ATG5 Antibody (Clone#GCD-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

APG5; APG5 LIKE; APG5L; Apoptosis specific protein; ASP; ATG5; Autophagy protein 5; hAPG5

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

ATG5

克隆号

GCD-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ATG5

计算分子量

32 kDa

实际分子量

55 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Uniprot ID

Q9H1Y0

文献引用格式

ATG5 Antibody (Clone#GCD-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4603)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Autophagy protein 5

实验方案>

实验步骤

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