Anti-SRC Antibody (Clone#III-19)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IHC FCM

BM4873

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SRC antibody (BM4873). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SRC antigen affinity purified monoclonal antibody (BM4873) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SRC at approximately 60 kDa. The expected band size for SRC is at 60 kDa.
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Immunofluorescent analysis using the Antibody.
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产品简介>

产品名称

Anti-SRC Antibody (Clone#III-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ASV; c SRC; p60 Src; pp60c src; Proto oncogene c Src; SRC; SRC1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IHC, FCM

基因名称

SRC

克隆号

III-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Src

计算分子量

60 kDa

实际分子量

60 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Sarcoma (Scr) is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human SRC protooncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.

Uniprot ID

P12931

文献引用格式

SRC Antibody (Clone#III-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4873)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Proto-oncogene tyrosine-protein kinase Src

实验方案>

实验步骤

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