Western blot (WB): | 1:500-2000 |
Western blot analysis of anti-SRC antibody (BA1404-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat PC-12whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (BA1404-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SRC at approximately 60 kDa. The expected band size for SRC is at 60 kDa.
Western blot analysis of anti-SRC antibody (BA1404-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat PC-12whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (BA1404-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SRC at approximately 60 kDa. The expected band size for SRC is at 60 kDa.