Anti-NFkB/NFKB2 p100/p52 Antibody

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP

BM5404

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-NFkB/NFKB2 antibody (BM5404). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFkB/NFKB2 antigen affinity purified monoclonal antibody (BM5404) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFkB/NFKB2 at approximately 110 kDa. The expected band size for NFkB/NFKB2 is at 97 kDa.
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产品简介>

产品名称

Anti-NFkB/NFKB2 p100/p52 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

DNA binding factor KBF2; H2TF1; LYT 10; LYT10; NF kappa B; NFKB2; NFKB2; p100; Oncogene Lyt 10; p100

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IP

基因名称

NFKB2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NFkB/NFKB2 p100/p52.

计算分子量

97 kDa

实际分子量

110 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.

Uniprot ID

Q00653

文献引用格式

NFkB/NFKB2 p100/p52 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM5404)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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