Anti-NFkB/NFKB2 p100/p52 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9150

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-NFkB/NFKB2 p100/p52 antibody (PB9150). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFkB/NFKB2 p100/p52 antigen affinity purified polyclonal antibody (PB9150) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFkB/NFKB2 p100/p52 at approximately 52 kDa (active form), 120kDa (precursor). The expected band size for NFkB/NFKB2 p100/p52 is at 97 kDa.
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Figure 2. IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(5)
Figure 3. IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(5)
Figure 4. IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(5)
Figure 5. IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150).
NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(5)

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产品简介>

产品名称

Anti-NFkB/NFKB2 p100/p52 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

DNA binding factor KBF2; H2TF1; LYT 10; LYT10; NF kappa B; NFKB2; NFKB2; p100; Oncogene Lyt 10; p100

产品类型

Polyclonal

检验物种

human,mouse,rat

应用范围

WB,IHC

基因名称

NFKB2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human NFkB/NFKB2 p100/p52 recombinant protein (Position: M1-R340). Human NFkB/NFKB2 p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB/NFKB2 p100/p52.

计算分子量

97 kDa

实际分子量

52 kDa(activeform)/120 kDa(precursor)

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB). NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.

Uniprot ID

Q00653

RRID

AB_3081991

文献引用格式

NFkB/NFKB2 p100/p52 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9150)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

蛋白名全称

Nuclear factor NF-kappa-B p100 subunit

实验方案>

实验步骤

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