Human Amphiregulin/AREG ELISA Kit

96T/2480

amphiregulin; AR; AREG; AREGB; CRDGF; SDGF

Expression system for standard: E.coli; Immunogen sequence: S101-K198

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

15.6 pg/ml - 1000 pg/ml

AREG

Amphiregulin

P15514

Human Amphiregulin/AREG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0304)

Amphiregulin, also known as AREG, is a protein that is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to mouse chromosome 5. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

25-30分钟

Catalog number	Description	Quantity
EK0304-CAP	Anti-Human Amphiregulin Pre-coated 96-well strip microplate	1
EK0304-ST	Human Amphiregulin Standard	10ng/tube×2
EK0304-DA	Human Amphiregulin Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	28	200	441	30	197	433
Standard deviation	1.65	10.4	29.54	2.19	10.63	31.6
CV(%)	6.7	5.2	6.7	7.3	5.4	7.3

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.016	0.077	0.147	0.253	0.571	0.840	1.824	2.587

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Human Milk

3.5h