产品名称
Mouse Amphiregulin/AREG ELISA Kit
指标别名
amphiregulin; AR; AREG; AREGB; CRDGF; SDGF
免疫原
Expression system for standard: E.coli; Immunogen sequence: V100-A248
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Amphiregulin/AREG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0591)
背景资料
Amphiregulin, also known as AREG, is a protein that is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to mouse chromosome 5. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0591-CAP | 96-well plate precoated with anti- mouse Amphiregulin antibody | 1 |
EK0591-ST | Lyophilized recombinant mouse Amphiregulin standard | 10ng/tubex2 |
EK0591-DA | Biotinylated anti- mouse Amphiregulin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 31 | 206 | 409 | 33 | 191 | 397 |
Standard deviation | 2.01 | 9.27 | 19.22 | 2.14 | 12.22 | 26.2 |
CV(%) | 6.5% | 4.5% | 4.7% | 6.5% | 6.4% | 6.6% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.038 | 0.121 | 0.212 | 0.410 | 0.746 | 1.231 | 1.625 | 1.866 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)