Human CD95/FAS ELISA Kit

96T/1880

ALPS1A; APO 1; Apo 1 antigen; APT1; CD95; FAS; FAS/CD95; FAS1; FASLG receptor; FASTM; TNFRSF6

NSO, R17-N173

There is no detectable cross-reactivity with other relevant proteins.

<3 pg/ml

31.2 pg/ml - 2000 pg/ml

FAS

Fas cell surface death receptor

P25445  

Human CD95/FAS ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0335)

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.

25-30分钟

Catalog number	Description	Quantity
EK0335-CAP	96-well plate precoated with anti- human FAS antibody	1
EK0335-ST	Lyophilized recombinant human FAS standard	10ng/tube×2
EK0335-DA	Biotinylated anti- human FAS antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	68	235	915	63	239	861
Standard deviation	3.6	14.8	44.83	3.33	18.88	54.24
CV(%)	5.3	6.3	4.9	5.3	7.9	6.3

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.012	0.061	0.108	0.193	0.359	0.639	1.172	1.756

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h