Mouse CD95/FAS ELISA Kit

96T/2480

ALPS1A; APO 1; Apo 1 antigen; APT1; CD95; FAS; FAS/CD95; FAS1; FASLG receptor; FASTM; TNFRSF6

Expression system for standard: NS0; Immunogen sequence: Q22-R169

There is no detectable cross-reactivity with other relevant proteins.

<3pg/ml

31.2pg/ml-2000pg/ml

FAS

Fas cell surface death receptor

Q8C350  

Mouse CD95/FAS ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0336)

Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.

Catalog number	Description	Quantity
EK0336-CAP	Anti-Mouse Fas Pre-coated 96-well strip microplate	1
EK0336-ST	Mouse Fas Standard	10ng/tube×2
EK0336-DA	Mouse Fas Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	67	227	869	60	222	905
Standard deviation	3.35	14.07	63.43	3.66	17.76	70.59
CV(%)	5%	6.2%	7.3%	6.1%	8%	7.8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.032	0.123	0.183	0.327	0.593	0.983	1.574	2.081

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h