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Human FASL/FASLG ELISA Kit

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EK0337

  • 96T ¥2280
  • 货期: 现货

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产品名称
Human FASL/FASLG ELISA Kit
规格/价格
96T/2280
指标别名
Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; Fas antigen ligand; Fas ligand; FASL; FASLG; TNFSF6
免疫原
CHO, P134-L281
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
FASLG
基因名全称
Fas ligand
Uniprot ID
P48023  
文献引用格式
Human FASL/FASLG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0337)
背景资料
Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0337-CAP96-well plate precoated with anti- human FASL antibody1
EK0337-STLyophilized recombinant human FASL standard10ng/tube×2
EK0337-DABiotinylated anti- human FASL antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3618643734189419
Standard deviation2.199.6728.842.112.4733.1
CV(%)6.15.26.66.26.67.9
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0080.1140.1960.3790.7401.3712.1212.693
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
实验时长
3.5h

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