产品名称
Human FASL/FASLG ELISA Kit
指标别名
Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; Fas antigen ligand; Fas ligand; FASL; FASLG; TNFSF6
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human FASL/FASLG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0337)
背景资料
Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0337-CAP | 96-well plate precoated with anti- human FASL antibody | 1 |
EK0337-ST | Lyophilized recombinant human FASL standard | 10ng/tube×2 |
EK0337-DA | Biotinylated anti- human FASL antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 186 | 437 | 34 | 189 | 419 |
Standard deviation | 2.19 | 9.67 | 28.84 | 2.1 | 12.47 | 33.1 |
CV(%) | 6.1 | 5.2 | 6.6 | 6.2 | 6.6 | 7.9 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.008 | 0.114 | 0.196 | 0.379 | 0.740 | 1.371 | 2.121 | 2.693 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)