产品名称
Mouse FASL/FASLG ELISA Kit
指标别名
Apoptosis antigen ligand; APT1LG1; APTL; CD178; CD95 L; CD95 ligand; CD95L; Fas antigen ligand; Fas ligand; FASL; FASLG; TNFSF6
免疫原
Expression system for standard: NS0; Immunogen sequence: P132-L279
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor ligand superfamily member 6
文献引用格式
Mouse FASL/FASLG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0338)
背景资料
FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0338-CAP | 96-well plate precoated with anti- mouse FAS-L antibody | 1 |
EK0338-ST | Lyophilized recombinant mouse FAS-L standard | 10ng/tube×2 |
EK0338-DA | Biotinylated anti- mouse FAS-L antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 68 | 206 | 772 | 65 | 216 | 727 |
Standard deviation | 2.99 | 13.59 | 49.4 | 3.51 | 17.28 | 47.98 |
CV(%) | 4.4% | 6.6% | 6.4% | 5.4% | 8% | 6.6% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.016 | 0.074 | 0.127 | 0.232 | 0.456 | 0.863 | 1.521 | 2.210 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)