产品名称
Human IGF1 ELISA Kit
指标别名
IBP1; IGF I; IGF1; IGF-1; IGF1A; IGF1B; IGFI; Insulin like growth factor I; Mechano growth factor; MGF; Somatomedin C
交叉反应
There is no detectable cross-reactivity with IGF-2.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
insulin like growth factor 1
文献引用格式
Human IGF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0376)
背景资料
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0376-CAP | 96-well plate precoated with anti- human IGF-1 antibody | 1 |
EK0376-ST | Lyophilized recombinant human IGF-1 standard | 10ng/tube×2 |
EK0376-DA | Biotinylated anti- human IGF-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 215 | 1065 | 3566 | 284 | 1154 | 4861 |
Standard deviation | 8.82 | 45.80 | 164.04 | 14.48 | 68.09 | 325.69 |
CV(%) | 4.1 | 4.3 | 4.6 | 5.1 | 5.9 | 6.7 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.053 | 0.141 | 0.199 | 0.325 | 0.502 | 0.914 | 1.377 | 1.992 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)