Chinese Hamster IGF1 ELISA Kit

96T/2280

IBP1; IGF I; IGF1; IGF-1; IGF1A; IGF1B; IGFI; Insulin like growth factor I; Mechano growth factor; MGF; Somatomedin C

Expression system for standard: E.coli; Immunogen sequence: G49-A118

There is detectable cross-reactivity with IGF-2< 1%.

<5pg/ml

62.5pg/ml-4000pg/ml

Igf1

Insulin-like growth factor I

P08025  

Chinese Hamster IGF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0377-HA)

Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Chinese hamster IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

20-25分钟

Catalog number	Description	Quantity
EK0377-HA-CAP	Anti-Hamster Igf1 Pre-coated 96-well strip microplate	1
EK0377-HA-ST	Hamster Igf1 Standard	10ng/tube×2
EK0377-HA-DA	Hamster Igf1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	527	1441	2641	497	1532	2737
Standard deviation	32.67	56.20	140	41.25	82.73	156
CV (%)	6.2	3.9	5.3	8.3	5.4	5.7

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.023	0.080	0.141	0.258	0.609	1.233	1.634	2.085

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

hamster

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)