指标别名
EDF; IL 5; IL5; IL-5; Interleukin 5; T cell replacing factor; TRF
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
文献引用格式
Human IL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0407)
背景资料
Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0407-CAP | Anti-Human IL-5 Pre-coated 96-well strip microplate | 1 |
EK0407-ST | Human IL-5 Standard | 10ng/tube×2 |
EK0407-DA | Human IL-5 Biotinylated antibody (100x) | 100µl |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100µl |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 9 | 73 | 238 | 8 | 70 | 234 |
Standard deviation | 0.7 | 4.67 | 16.42 | 0.74 | 4.55 | 20.12 |
CV(%) | 7.8 | 6.4 | 6.9 | 9.3 | 6.5 | 8.6 |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.005 | 0.078 | 0.150 | 0.269 | 0.501 | 0.862 | 1.545 | 2.201 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine