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Human IL5 ELISA Kit

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EK0407

  • 96T ¥1880
  • 货期: 现货

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产品名称
Human IL5 ELISA Kit
规格/价格
96T/1880
指标别名
EDF; IL 5; IL5; IL-5; Interleukin 5; T cell replacing factor; TRF
免疫原
sf21, I20-S134
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<2 pg/ml
检测范围
7.8 pg/ml - 500 pg/ml
基因名称
IL5
基因名全称
interleukin 5
Uniprot ID
P05113  
文献引用格式
Human IL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0407)
背景资料
Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0407-CAPAnti-Human IL-5 Pre-coated 96-well strip microplate1
EK0407-STHuman IL-5 Standard10ng/tube×2
EK0407-DAHuman IL-5 Biotinylated antibody (100x)100µl
AR1103Avidin-Biotin-Peroxidase Complex (100x)100µl
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)973238870234
Standard deviation0.74.6716.420.744.5520.12
CV(%)7.86.46.99.36.58.6
典型数据示例
Concentration(pg/ml)07.815.631.362.5125250500
O.D.0.0050.0780.1500.2690.5010.8621.5452.201
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine
实验时长
3.5h

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