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Mouse IL5 ELISA Kit

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EK0408

  • 96T ¥1880
  • 货期: 现货

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产品名称
Mouse IL5 ELISA Kit
规格/价格
96T/1880
指标别名
EDF; IL 5; IL5; IL-5; Interleukin 5; T cell replacing factor; TRF
免疫原
Expression system for standard: NS0; Immunogen sequence: M21-G133
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<2pg/ml
检测范围
15.6pg/ml-1000pg/ml
基因名称
IL5
基因名全称
Interleukin-5
Uniprot ID
P04401  
文献引用格式
Mouse IL5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0408)
背景资料
Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
试剂盒内容
Catalog numberDescriptionQuantity
EK0408-CAP96-well plate precoated with anti- mouse IL-5 antibody1
EK0408-STLyophilized recombinant mouse IL-5 standard10ng/tubex2
EK0408-DABiotinylated anti- mouse IL-5 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3622045334214478
Standard deviation2.529.2421.292.5810.730.59
CV(%)7%4.2%4.7%7.6%5%6.4%
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0250.0830.1440.2580.4670.8821.7542.352
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants and Serum
实验时长
3.5h

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