Human MCP-1/CCL2 ELISA Kit

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EK0441

96T ￥2480
货期：现货

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Human MCP-1 ELISA Kit standard curve
all(2)
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产品简介>

产品名称

Human MCP-1/CCL2 ELISA Kit

规格/价格

96T/2480

指标别名

C C motif chemokine 2; CCL2; chemokine (C C motif) ligand 2; GDCF 2; HC11; HSMCR30; MCAF; MCP 1; MCP1; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2; SMC CF

免疫原

E.coli, Q24-T99

交叉反应

There is cross-reactivity with human Eotaxin, MCP-3<1%.

敏感性

<1 pg/ml

检测范围

15.6 pg/ml - 1000 pg/ml

基因名称

CCL2

基因名全称

chemokine (C-C motif) ligand 2

Uniprot ID

P13500

文献引用格式

Human MCP-1/CCL2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0441)

背景资料

Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0441-CAP	96-well plate precoated with anti- human MCP-1 antibody	1
EK0441-ST	Lyophilized recombinant human MCP-1 standard	10ng/tubex2
EK0441-DA	Biotinylated anti- human MCP-1 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	31	159	477	30	148	481
Standard deviation	1.55	8.74	32.91	1.98	9.76	37.51
CV(%)	5	5.5	6.9	6.6	6.6	7.8

典型数据示例

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.008	0.100	0.206	0.426	0.826	1.271	1.762	2.087

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human MCP-1/CCL2 ELISA Kit

EK0441

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

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Human MCP-1/CCL2 ELISA Kit

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