产品名称
Mouse MCP-1/CCL2 ELISA Kit
指标别名
C C motif chemokine 2; CCL2; chemokine (C C motif) ligand 2; GDCF 2; HC11; HSMCR30; MCAF; MCP 1; MCP1; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2; SMC CF
免疫原
Expression system for standard: E.coli; Immunogen sequence: Q24-R96
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 2
文献引用格式
Mouse MCP-1/CCL2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0568)
背景资料
Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0568-CAP | 96-well plate precoated with anti- mouse MCP-1 antibody | 1 |
| EK0568-ST | Lyophilized recombinant mouse MCP-1 standard | 10ng/tubex2 |
| EK0568-DA | Biotinylated anti- mouse MCP-1 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 39 | 224 | 394 | 40 | 231 | 398 |
| Standard deviation | 1.56 | 10.75 | 27.18 | 2 | 12.47 | 27.46 |
| CV(%) | 4% | 4.8% | 6.9% | 5% | 5.4% | 6.9% |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.008 | 0.103 | 0.214 | 0.392 | 0.806 | 1.398 | 1.953 | 2.290 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum
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