产品名称
Human TIE2/TEK ELISA Kit
指标别名
Angiopoietin 1 receptor; CD202B; hTIE2; p140 TEK; TEK; TIE 2; Tie2; VMCM; VMCM1
交叉反应
There is cross-reactivates with human TIE1< 0.1%.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
TEK tyrosine kinase, endothelial
文献引用格式
Human TIE2/TEK ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0519)
背景资料
Tyrosine kinase with Ig and EGF homology domain 2(Tie-2), also called TEK tyrosine kinase, endothelial(TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0519-CAP | 96-well plate precoated with anti- human TIE2 antibody | 1 |
EK0519-ST | Lyophilized recombinant human TIE2 standard | 10ng/tubex2 |
EK0519-DA | Biotinylated anti- human TIE2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 305 | 1860 | 4704 | 311 | 1714 | 4949 |
Standard deviation | 23.79 | 81.84 | 263.42 | 27.05 | 99.41 | 351.37 |
CV(%) | 7.8 | 4.4 | 5.6 | 8.7 | 5.8 | 7.1 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.021 | 0.075 | 0.120 | 0.231 | 0.430 | 0.888 | 1.623 | 2.371 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)