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Human TIE2/TEK ELISA Kit

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EK0519

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human TIE2/TEK ELISA Kit
规格/价格
96T/2480
指标别名
Angiopoietin 1 receptor; CD202B; hTIE2; p140 TEK; TEK; TIE 2; Tie2; VMCM; VMCM1
免疫原
NSO, A23-K745
交叉反应
There is cross-reactivates with human TIE1< 0.1%.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
TEK
基因名全称
TEK tyrosine kinase, endothelial
Uniprot ID
Q02763  
文献引用格式
Human TIE2/TEK ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0519)
背景资料
Tyrosine kinase with Ig and EGF homology domain 2(Tie-2), also called TEK tyrosine kinase, endothelial(TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0519-CAP96-well plate precoated with anti- human TIE2 antibody1
EK0519-STLyophilized recombinant human TIE2 standard10ng/tubex2
EK0519-DABiotinylated anti- human TIE2 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3051860470431117144949
Standard deviation23.7981.84263.4227.0599.41351.37
CV(%)7.84.45.68.75.87.1
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0210.0750.1200.2310.4300.8881.6232.371
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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