产品名称
Mouse TIE2/TEK ELISA Kit
指标别名
Angiopoietin 1 receptor; CD202B; hTIE2; p140 TEK; TEK; TIE 2; Tie2; VMCM; VMCM1
免疫原
Expression system for standard: NS0; Immunogen sequence: A23-K744
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Angiopoietin-1 receptor
文献引用格式
Mouse TIE2/TEK ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0976)
背景资料
Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
试剂盒内容
Catalog number | Description | Quantity |
EK0976-CAP | 96-well plate precoated with anti- mouse TEK/TIE2 antibody | 1 |
EK0976-ST | Lyophilized recombinant mouse TEK/TIE2 standard | 10ng/tubex2 |
EK0976-DA | Biotinylated anti- mouse TEK/TIE2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 158 | 1232 | 4836 | 145 | 1295 | 4385 |
Standard deviation | 7.9 | 66.52 | 203.11 | 7.97 | 90.65 | 210.48 |
CV(%) | 5% | 5.4% | 4.2% | 5.5% | 7% | 4.8% |
典型数据示例
Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
O.D. | 0.038 | 0.066 | 0.100 | 0.160 | 0.299 | 0.568 | 1.193 | 2.153 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)