产品名称
Human TIMP1 ELISA Kit
指标别名
CLGI; Collagenase inhibitor; EPA; EPO; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP 1; TIMP1; TIMP-1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
TIMP metallopeptidase inhibitor 1
文献引用格式
Human TIMP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0520)
背景资料
The tissue inhibitor of metalloproteinases 1(TIMP1) is also called erythroid-potentiating activity(EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3(IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0520-CAP | 96-well plate precoated with anti- human TIMP-1 antibody | 1 |
EK0520-ST | Lyophilized human TIMP-1 standard | 10ng/tubex2 |
EK0520-DA | Biotinylated anti- human TIMP-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 277 | 1942 | 4295 | 285 | 2099 | 4333 |
Standard deviation | 16.34 | 122.34 | 292.06 | 21.66 | 146.93 | 363.97 |
CV(%) | 5.9 | 6.3 | 6.8 | 7.6 | 7 | 8.4 |
典型数据示例
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.003 | 0.438 | 0.759 | 1.197 | 1.322 | 1.669 | 1.805 | 2.033 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Saliva