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Human TIMP1 ELISA Kit

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EK0520

  • 96T ¥2280
  • 货期: 现货

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产品名称
Human TIMP1 ELISA Kit
规格/价格
96T/2280
指标别名
CLGI; Collagenase inhibitor; EPA; EPO; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP 1; TIMP1; TIMP-1
免疫原
NSO, C24-A207
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<5 pg/ml
检测范围
15.6pg/ml-1000pg/ml
基因名称
TIMP1
基因名全称
TIMP metallopeptidase inhibitor 1
Uniprot ID
P01033  
文献引用格式
Human TIMP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0520)
背景资料
The tissue inhibitor of metalloproteinases 1(TIMP1) is also called erythroid-potentiating activity(EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3(IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0520-CAP96-well plate precoated with anti- human TIMP-1 antibody1
EK0520-STLyophilized human TIMP-1 standard10ng/tubex2
EK0520-DABiotinylated anti- human TIMP-1 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2771942429528520994333
Standard deviation16.34122.34292.0621.66146.93363.97
CV(%)5.96.36.87.678.4
典型数据示例
Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0030.4380.7591.1971.3221.6691.8052.033
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Saliva
实验时长
3.5h

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