产品名称
Mouse TIMP1 ELISA Kit
指标别名
CLGI; Collagenase inhibitor; EPA; EPO; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP 1; TIMP1; TIMP-1
免疫原
Expression system for standard: NS0; Immunogen sequence: C25-R205
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Metalloproteinase inhibitor 1
文献引用格式
Mouse TIMP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0521)
背景资料
TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. It was found to reside at Xp11.4-p11.1. This is the first growth factor found to be X-linked. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. What’s more, TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0521-CAP | 96-well plate precoated with anti- mouse TIMP-1 antibody | 1 |
EK0521-ST | Lyophilized recombinant mouse TIMP-1 standard | 10ng/tube×2 |
EK0521-DA | Biotinylated anti- mouse TIMP-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 65 | 258 | 787 | 62 | 246 | 710 |
Standard deviation | 3.44 | 16.51 | 41.71 | 4.21 | 20.41 | 45.44 |
CV(%) | 5.3% | 6.4% | 5.3% | 6.8% | 8.3% | 6.4% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.004 | 0.097 | 0.186 | 0.374 | 0.638 | 1.117 | 1.734 | 2.522 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)