Human uPAR/PLAUR ELISA Kit

96T/2480

CD87; MO3; PLAUR; U PAR; UPAR; URKR

NSO,L23-R303

There is no detectable cross-reactivity with other relevant proteins.

<4 pg/ml

62.5 pg/ml - 4000 pg/ml

PLAUR

plasminogen activator, urokinase receptor

Q03405  

Human uPAR/PLAUR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0536)

The urokinase-type plasminogen activator receptor(uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31KDa.

20-25分钟

Catalog number	Description	Quantity
EK0536-CAP	96-well plate precoated with anti- human uPAR antibody	1
EK0536-ST	Lyophilized recombinant human uPAR standard	10ng/tubex2
EK0536-DA	Biotinylated anti- human uPAR antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	162	487	1379	146	478	1361
Standard deviation	10.69	37.49	66.19	12.11	37.28	85.74
CV(%)	6.6	7.7	4.8	8.3	7.8	6.3

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.004	0.042	0.090	0.178	0.384	0.744	1.505	2.317

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine

3.5h