产品名称
Mouse uPAR/PLAUR ELISA Kit
指标别名
CD87; MO3; PLAUR; U PAR; UPAR; URKR
免疫原
Expression system for standard: NS0; Immunogen sequence: L24-T297
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Urokinase plasminogen activator surface receptor
文献引用格式
Mouse uPAR/PLAUR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1215)
背景资料
The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1215-CAP | 96-well plate precoated with anti- mouse uPAR antibody | 1 |
EK1215-ST | Lyophilized recombinant mouse uPAR standard | 10ng/tube×2 |
EK1215-DA | Biotinylated anti- mouse uPAR antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 102 | 569 | 1345 | 111 | 532 | 1266 |
Standard deviation | 7.95 | 40.39 | 71.28 | 10.21 | 42.02 | 81.02 |
CV(%) | 7.8% | 7.1% | 5.3% | 9.2% | 7.9% | 6.4% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.053 | 0.117 | 0.177 | 0.278 | 0.452 | 0.778 | 1.347 | 1.946 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)