Human CCL21 ELISA Kit

96T/2480

C-C motif chemokine ligand 21; 6Ckine; CKb9; ECL; SCYA21; SLC; TCA4; CCL21

E.coli, S24-P134

There is no detectable cross-reactivity with other relevant proteins.

<12 pg/ml

62.5 pg/ml - 4000 pg/ml

CCL21

chemokine (C-C motif) ligand 21

O00585

Human CCL21 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0555)

Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

15-20分钟

Catalog number	Description	Quantity
EK0555-CAP	96-well plate precoated with anti- human CCL21 antibody	1
EK0555-ST	Lyophilized recombinant human CCL21 standard	10ng/tubex2
EK0555-DA	Biotinylated anti- human CCL21 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	100	709	1352	103	706	1475
Standard deviation	5.6	28.36	82.47	6.9	31.77	92.92
CV(%)	5.6	4	6.1	6.7	4.5	6.3

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.031	0.057	0.102	0.203	0.408	0.504	1.178	2.318

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h