Human CCL21 Quick ELISA Kit

96T/3580

C-C motif chemokine ligand 21; 6Ckine; CKb9; ECL; SCYA21; SLC; TCA4; CCL21

Expression system for standard: E.coli; Immunogen sequence: S24-P134

There is no detectable cross-reactivity with other relevant proteins.

<12pg/ml

62.5pg/ml-4000pg/ml

CCL21

chemokine (C-C motif) ligand 21

O00585

Human CCL21 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0555)

Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

15分钟

Catalog number	Description	Quantity
FEK0663-CAP	96-well plate precoated with anti-Human CCL21 antibody	1
FEK0663-ST	lyophilized recombinant Human CCL21 standard	10ng/tube×2
FEK0663-A	Human CCL21 Antibody A	3ml
FEK0555-B	Human CCL21 Antibody B	3ml
AR1106-1	Sample Diluent	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
PLA-SEA	Adhesive Plate Sealers	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	117	825	1412	108	794	1510
Standard deviation	4.56	30.53	83.31	7.02	34.94	92.11
CV (%)	3.9%	3.7%	5.9%	6.5%	4.4%	6.1%

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.010	0.139	0.313	0.475	0.802	1.584	2.396	2.608

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)