Mouse Amphiregulin/AREG ELISA Kit

96T/2480

amphiregulin; AR; AREG; AREGB; CRDGF; SDGF

Expression system for standard: E.coli; Immunogen sequence: V100-A248

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

15.6pg/ml-1000pg/ml

AREG

Amphiregulin

P31955

Mouse Amphiregulin/AREG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0591)

Amphiregulin, also known as AREG, is a protein that is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to mouse chromosome 5. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

Catalog number	Description	Quantity
EK0591-CAP	96-well plate precoated with anti- mouse Amphiregulin antibody	1
EK0591-ST	Lyophilized recombinant mouse Amphiregulin standard	10ng/tubex2
EK0591-DA	Biotinylated anti- mouse Amphiregulin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	31	206	409	33	191	397
Standard deviation	2.01	9.27	19.22	2.14	12.22	26.2
CV(%)	6.5%	4.5%	4.7%	6.5%	6.4%	6.6%

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.038	0.121	0.212	0.410	0.746	1.231	1.625	1.866

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h