Catalog number | Description | Quantity |
---|---|---|
EK0657-CAP | 96-well plate precoated with anti- human Angiopoietin-2 antibody | 1 |
EK0657-ST | Lyophilized recombinant human Angiopoietin-2 standard | 10ng/tubex2 |
EK0657-DA | Biotinylated anti- human Angiopoietin-2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 327 | 1634 | 5900 | 341 | 1769 | 5438 |
Standard deviation | 17.33 | 114.38 | 354 | 23.52 | 127.36 | 342.59 |
CV(%) | 5.3 | 7 | 6 | 6.9 | 7.2 | 6.3 |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.095 | 0.178 | 0.210 | 0.376 | 0.519 | 0.970 | 1.588 | 2.010 |
Human Angiopoietin-2 ELISA Kit standard curve
Human Angiopoietin-2 ELISA Kit standard curve