Human Angiopoietin-2/ANGPT2 ELISA Kit

说明书

EK0657

96T ￥2480
货期：现货

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产品简介>

产品名称

Human Angiopoietin-2/ANGPT2 ELISA Kit

规格/价格

96T/2480

指标别名

AGPT2; ANG 2; ANG2; angiopoietin 2; Angiopoietin-2; ANGPT2; PDGFAB

免疫原

NSO, Y19-F496

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10 pg/ml

检测范围

156 pg/ml - 10000 pg/ml

基因名称

ANGPT2

基因名全称

angiopoietin 2

Uniprot ID

O15123

文献引用格式

Human Angiopoietin-2/ANGPT2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0657)

背景资料

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0657-CAP	96-well plate precoated with anti- human Angiopoietin-2 antibody	1
EK0657-ST	Lyophilized recombinant human Angiopoietin-2 standard	10ng/tubex2
EK0657-DA	Biotinylated anti- human Angiopoietin-2 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	327	1634	5900	341	1769	5438
Standard deviation	17.33	114.38	354	23.52	127.36	342.59
CV(%)	5.3	7	6	6.9	7.2	6.3

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.095	0.178	0.210	0.376	0.519	0.970	1.588	2.010

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

应用范围

ELISA

检验物种

human

检测方法

酶联免疫双抗夹心法

样本类型

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Human Milk

实验时长

3.5h

实验方案>

实验步骤

Human Angiopoietin-2/ANGPT2 ELISA Kit

EK0657

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

实验方案>

引用文献(0)>

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