产品名称
Rat Angiopoietin-2/ANGPT2 ELISA Kit
指标别名
AGPT2; ANG 2; ANG2; angiopoietin 2; Angiopoietin-2; ANGPT2; PDGFAB
免疫原
Expression system for standard: NS0; Immunogen sequence: Y19-F496
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat Angiopoietin-2/ANGPT2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1574)
背景资料
Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
试剂盒内容
Catalog number | Description | Quantity |
EK1574-CAP | 96-well plate precoated with anti- rat Angiopoietin-2 antibody | 1 |
EK1574-ST | Lyophilized recombinant rat Angiopoietin-2 standard | 10ng/tube×2 |
EK1574-DA | Biotinylated anti- rat Angiopoietin-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 253 | 1986 | 5096 | 239 | 1814 | 4721 |
Standard deviation | 19.98 | 99.3 | 229.32 | 22.22 | 121.53 | 231.32 |
CV(%) | 7.9% | 5% | 4.5% | 9.3% | 6.7% | 4.9% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.032 | 0.085 | 0.143 | 0.248 | 0.423 | 0.816 | 1.359 | 2.231 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)