Mouse Cathepsin B/CTSB ELISA Kit

96T/2480

APP secretase; APPS; cathepsin B; Cathepsin B1; CPSB; CTSB

Expression system for standard: NS0; Immunogen sequence: H18-F339

There is no detectable cross-reactivity with other relevant proteins.

<5pg/ml

156pg/ml-10000pg/ml

CTSB

Cathepsin B

P10605  

Mouse Cathepsin B/CTSB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0671)

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

Catalog number	Description	Quantity
EK0671-CAP	96-well plate precoated with anti- mouse Cathepsin B antibody	1
EK0671-ST	Lyophilized recombinant mouse Cathepsin B standard	10ng/tubex2
EK0671-DA	Biotinylated anti- mouse Cathepsin B antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	242	1828	4749	229	1916	4534
Standard deviation	14.76	100.54	370.42	13.96	128.37	439.79
CV(%)	6.1%	5.5%	7.8%	6.1%	6.7%	9.7%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.021	0.126	0.233	0.379	0.665	1.091	1.714	2.128

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h