Human Cathepsin B/CTSB Quick ELISA Kit

96T/3580

APP secretase; APPS; cathepsin B; Cathepsin B1; CPSB; CTSB

Expression system for standard: NS0; Immunogen sequence: R18-I339

There is no detectable cross-reactivity with other relevant proteins.

<5pg/ml

156pg/ml-10000pg/ml

CTSB

cathepsin B

P07858  

Human Cathepsin B/CTSB Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0670)

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

15分钟

Catalog number	Description	Quantity
FEK0670-CAP	Anti-Pre-coated 96-well Strip Microplate	1
FEK0670-ST	Human Cathepsin B standard	10ng/tube×2
FEK0670-A	Human Cathepsin B Antibody A	3ml
FEK0670-B	Human Cathepsin B Antibody B	3ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	252	1856	4296	234	1923	4064
Standard deviation	17.64	129.92	326.49	18.25	148.07	377.95
CV(%)	7%	7%	7.6%	7.8%	7.7%	9.3%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.107	0.243	0.348	0.539	0.894	1.445	2.041	2.443

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)