Mouse Cystatin C/CST3 ELISA Kit

96T/2480

ARMD11; CST3; Cystatin 3; cystatin C; CYTC; Gamma trace; Post gamma globulin

Expression system for standard: NS0; Immunogen sequence: M1-A140

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

312pg/ml-20000pg/ml

CST3

Cystatin-C

P21460  

Mouse Cystatin C/CST3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0679)

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

Catalog number	Description	Quantity
EK0679-CAP	96-well plate precoated with anti- mouse Cystatin C antibody	1
EK0679-ST	Lyophilized recombinant mouse Cystatin C standard	20ng/tubex2
EK0679-DA	Biotinylated anti- mouse Cystatin C antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	437	2310	9815	464	2268	10497
Standard deviation	27.96	97.02	706.68	34.8	106.59	871.25
CV(%)	6.4%	4.2%	7.2%	7.5%	4.7%	8.3%

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.044	0.162	0.271	0.445	0.831	1.360	1.416	1.822

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine

3.5h