Human Cystatin C/CST3 Quick ELISA Kit

96T/3580

ARMD11; CST3; Cystatin 3; cystatin C; CYTC; Gamma trace; Post gamma globulin

Expression system for standard: NS0; Immunogen sequence: M1-A146

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

0.312ng/ml-20ng/ml

CST3

cystatin C

P01034  

Human Cystatin C/CST3 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0678)

Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

15分钟

Catalog number	Description	Quantity
FEK0678-CAP	Anti-human CystatinC Pre-coated 96-well Strip Microplate	1
FEK0678-ST	human CystatinC standard	10ng/tube×2
FEK0678-H	HRP Linked Anti-human CystatinC Antibody	6ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	687	2837	10374	647	2715	10112
Standard deviation	43.96	207.1	684.68	51.11	217.2	859.52
CV(%)	6.4%	7.3%	6.6%	7.9%	8%	8.5%

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.034	0.102	0.153	0.267	0.469	0.870	1.441	2.180

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk