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Human B7-1/CD80 ELISA Kit

说明书

EK0707

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human B7-1/CD80 ELISA Kit
规格/价格
96T/2480
指标别名
Activation B7 1 antigen; B7; B7 1; B7-1; BB1; CD28LG; CD28LG1; CD80; CD80 molecule; CTLA 4 counter receptor B7.1; LAB7
免疫原
NSO, V35-N242
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10 pg/ml
检测范围
62.5 pg/ml - 4000 pg/ml
基因名称
CD80
基因名全称
CD80 molecule
Uniprot ID
P33681  
文献引用格式
Human B7-1/CD80 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0707)
背景资料
The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0707-CAP96-well plate precoated with anti- human CD80 antibody1
EK0707-STLyophilized recombinant human CD80 standard10ng/tube×2
EK0707-DABiotinylated anti- human CD80 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)16244516561634251791
Standard deviation7.2934.7169.557.9834100.29
CV(%)4.57.84.24.985.6
典型数据示例
Concentration(pg/ml)062.5125250500100020004000
O.D.0.0060.0600.1290.2560.4420.8291.3841.979
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants and Serum
实验时长
3.5h

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