Mouse B7-1/CD80 ELISA Kit

说明书

EK0708

96T ￥2480
货期：现货

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Mouse B7-1/CD80 ELISA Kit standard curve
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产品简介>

产品名称

Mouse B7-1/CD80 ELISA Kit

规格/价格

96T/2480

指标别名

Activation B7 1 antigen; B7; B7 1; B7-1; BB1; CD28LG; CD28LG1; CD80; CD80 molecule; CTLA 4 counter receptor B7.1; LAB7

免疫原

Expression system for standard: NS0; Immunogen sequence: D37-K245

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10pg/ml

检测范围

62.5pg/ml-4000pg/ml

基因名称

CD80

基因名全称

T-lymphocyte activation antigen CD80

Uniprot ID

Q00609

文献引用格式

Mouse B7-1/CD80 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0708)

背景资料

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1, D37—K245, which is composed of two single chains acids with the dipolymer.

试剂盒内容

Catalog number	Description	Quantity
EK0708-CAP	96-well plate precoated with anti- mouse CD80 antibody	1
EK0708-ST	Lyophilized recombinant mouse CD80 standard	10ng/tube×2
EK0708-DA	Biotinylated anti- mouse CD80 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	153	733	1704	158	717	1630
Standard deviation	9.94	47.64	132.91	13.27	59.51	133.66
CV(%)	6.5%	6.5%	7.8%	8.4%	8.3%	8.2%

典型数据示例

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.001	0.082	0.174	0.333	0.609	0.997	1.508	2.112

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse B7-1/CD80 ELISA Kit

EK0708

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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