产品名称
Human Galectin-3/LGALS3 ELISA Kit
指标别名
35 kDa lectin; CBP 35; CBP35; Gal 3; GAL3; Galactose specific lectin 3; Galactoside binding protein; GALBP; Galectin 3; Galectin-3; GALIG; IgE binding protein; L 31; Laminin binding protein; Lectin L 29; LGALS2; LGALS3; Mac 2 antigen; MAC2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
lectin, galactoside-binding, soluble, 3
文献引用格式
Human Galectin-3/LGALS3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0764)
背景资料
Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0764-CAP | 96-well plate precoated with anti- human Galectin-3 antibody | 1 |
EK0764-ST | Lyophilized recombinant human Galectin-3standard | 10ng/tube×2 |
EK0764-DA | Biotinylated anti- human Galectin-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 212 | 1876 | 4230 | 205 | 1951 | 4307 |
Standard deviation | 16.74 | 76.91 | 334.17 | 19.88 | 115.1 | 374.7 |
CV(%) | 7.9 | 4.1 | 7.9 | 9.7 | 5.9 | 8.7 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.013 | 0.117 | 0.201 | 0.405 | 0.746 | 1.191 | 1.680 | 2.010 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)