产品名称
Mouse Galectin-3/LGALS3 ELISA Kit
指标别名
35 kDa lectin; CBP 35; CBP35; Gal 3; GAL3; Galactose specific lectin 3; Galactoside binding protein; GALBP; Galectin 3; Galectin-3; GALIG; IgE binding protein; L 31; Laminin binding protein; Lectin L 29; LGALS2; LGALS3; Mac 2 antigen; MAC2
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-I264
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Galectin-3/LGALS3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0765)
背景资料
Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0765-CAP | 96-well plate precoated with anti- mouse Galectin-3antibody | 1 |
EK0765-ST | Lyophilized recombinant mouse Galectin-3 standard | 10ng/tube×2 |
EK0765-DA | Biotinylated anti- mouse Galectin-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 81 | 434 | 1619 | 80 | 402 | 1701 |
Standard deviation | 6.31 | 31.24 | 126.28 | 7.68 | 32.56 | 153.09 |
CV(%) | 7.8% | 7.2% | 7.8% | 9.6% | 8.1% | 9% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.020 | 0.057 | 0.093 | 0.167 | 0.311 | 0.629 | 1.164 | 1.979 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)