产品名称
Human Kallikrein 1/KLK1 ELISA Kit
指标别名
hK1; kallikrein 1; KLK1; KLKR; Tissue kallikrein
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Kallikrein 1/KLK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0903)
背景资料
Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0903-CAP | 96-well plate precoated with anti- human KLK1 antibody | 1 |
| EK0903-ST | Lyophilized recombinant human KLK1 standard | 10ng/tube×2 |
| EK0903-DA | Biotinylated anti- human KLK1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 304 | 1654 | 3697 | 280 | 1605 | 3442 |
| Standard deviation | 12.46 | 71.12 | 284.66 | 16.24 | 93.09 | 271.91 |
| CV(%) | 4.1 | 4.3 | 7.7 | 5.8 | 5.8 | 7.9 |
典型数据示例
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.016 | 0.084 | 0.157 | 0.322 | 0.513 | 0.965 | 1.516 | 2.020 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)