产品名称
Mouse Kallikrein 1/KLK1 ELISA Kit
指标别名
hK1; kallikrein 1; KLK1; KLKR; Tissue kallikrein
免疫原
Expression system for standard: NS0; Immunogen sequence: I25-D261
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Kallikrein 1/KLK1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1586)
背景资料
Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1586-CAP | 96-well plate precoated with anti- mouse KLK1 antibody | 1 |
| EK1586-ST | Lyophilized recombinant mouse KLK1 standard | 10ng/tube×2 |
| EK1586-DA | Biotinylated anti- mouse KLK1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 150 | 602 | 1476 | 154 | 580 | 1608 |
| Standard deviation | 9.15 | 43.94 | 116.6 | 11.08 | 51.62 | 136.68 |
| CV(%) | 6.1% | 7.3% | 7.9% | 7.2% | 8.9% | 8.5% |
典型数据示例
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.021 | 0.054 | 0.089 | 0.156 | 0.352 | 0.792 | 1.696 | 2.346 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)