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Human IL33 ELISA Kit

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EK0929

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human IL33 ELISA Kit
规格/价格
96T/2480
指标别名
C9orf26; DVS27; IL 1F11; IL 33; IL1F11; IL33; IL-33; Interleukin 1 family member 11; interleukin 33; NF HEV; NFEHEV; NFHEV; RP11 575C20.2
免疫原
E.coli, S112-T270
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10 pg/ml
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
IL33
基因名全称
interleukin 33
Uniprot ID
O95760  
文献引用格式
Human IL33 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0929)
背景资料
Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0929-CAP96-well plate precoated with anti- human IL-33 antibody1
EK0929-STLyophilized recombinant human IL-33 standard10ng/tube×2
EK0929-DABiotinylated anti- human IL-33 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3026048428282511
Standard deviation1.7416.3837.751.7622.2745.99
CV(%)5.86.37.86.37.99
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0290.0570.1010.1890.3350.6321.1522.098
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Cell Lysates
实验时长
3.5h

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