产品名称
Mouse IL33 ELISA Kit
指标别名
C9orf26; DVS27; IL 1F11; IL 33; IL1F11; IL33; IL-33; Interleukin 1 family member 11; interleukin 33; NF HEV; NFEHEV; NFHEV; RP11 575C20.2
免疫原
Expression system for standard: E.coli; Immunogen sequence: S109-I266
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL33 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0930)
背景资料
Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0930-CAP | 96-well plate precoated with anti- mouse IL-33 antibody | 1 |
EK0930-ST | Lyophilized recombinant mouse IL-33 standard | 10ng/tube×2 |
EK0930-DA | Biotinylated anti- mouse IL-33 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 63 | 290 | 939 | 67 | 300 | 883 |
Standard deviation | 4.72 | 22.62 | 44.13 | 5.22 | 27.6 | 50.33 |
CV(%) | 7.5% | 7.8% | 4.7% | 7.8% | 9.2% | 5.7% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.009 | 0.068 | 0.117 | 0.224 | 0.401 | 0.775 | 1.405 | 2.212 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)