产品名称
Rat TGF beta 2/TGFB2 ELISA Kit
指标别名
BSC 1 cell growth inhibitor; Cetermin; G TSF; Polyergin; TGF beta 2; TGF beta2; TGFB2; TGF-beta 2; TGFβ2; TGF-β2
免疫原
Expression system for standard: NS0; Immunogen sequence: A303-S414
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
transforming growth factor, beta 2
文献引用格式
Rat TGF beta 2/TGFB2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0982)
背景资料
Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
试剂盒内容
Catalog number | Description | Quantity |
EK0982-CAP | 96-well plate precoated with anti- rat TGF-beta 2 antibody | 1 |
EK0982-ST | Lyophilized recombinant rat TGF-beta 2 standard | 10ng/tubex2 |
EK0982-DA | Biotinylated anti- rat TGF-beta 2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 57 | 207 | 866 | 54 | 209 | 856 |
Standard deviation | 2.62 | 15.52 | 39.83 | 2.53 | 16.92 | 42.8 |
CV(%) | 4.6% | 7.5% | 4.6% | 4.7% | 8.1% | 5% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.028 | 0.052 | 0.082 | 0.148 | 0.248 | 0.605 | 1.281 | 1.911 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)