Mouse TGF beta 2/TGFB2 ELISA Kit

96T/2480

BSC 1 cell growth inhibitor; Cetermin; G TSF; Polyergin; TGF beta 2; TGF beta2; TGFB2; TGF-beta 2; TGFβ2; TGF-β2

Expression system for standard: NS0; Immunogen sequence: A303-S414

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

31.2pg/ml-2000pg/ml

TGFB2

Transforming growth factor beta-2

P27090

Mouse TGF beta 2/TGFB2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0983)

Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Catalog number	Description	Quantity
EK0983-CAP	96-well plate precoated with anti- mouse TGF-beta 2 antibody	1
EK0983-ST	Lyophilized recombinant mouse TGF-beta 2 standard	10ng/tubex2
EK0983-DA	Biotinylated anti- mouse TGF-beta 2 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	98	235	827	102	215	805
Standard deviation	6.46	11.98	54.58	7.54	11.82	64.4
CV(%)	6.6%	5.1%	6.6%	7.4%	5.5%	8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.055	0.094	0.160	0.260	0.536	0.996	1.749	2.527

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h