产品名称
Human ALCAM ELISA Kit
指标别名
ALCAM; CD166; CD166 antigen; MEMD
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
activated leukocyte cell adhesion molecule
文献引用格式
Human ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0995)
背景资料
CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0995-CAP | 96-well plate precoated with anti- human ALCAM antibody | 1 |
EK0995-ST | Lyophilized recombinant human ALCAM standard | 20ng/tube×2 |
EK0995-DA | Biotinylated anti- human ALCAM antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 648 | 1967 | 8283 | 688 | 2156 | 8924 |
Standard deviation | 37.58 | 145.55 | 563.24 | 48.16 | 198.35 | 651.45 |
CV(%) | 5.8 | 7.4 | 6.8 | 7 | 9.2 | 7.3 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.033 | 0.109 | 0.137 | 0.215 | 0.385 | 0.688 | 1.138 | 2.063 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)