Human ALCAM ELISA Kit

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EK0995

96T ￥2480
货期：现货

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Human ALCAM ELISA Kit standard curve
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产品简介>

产品名称

Human ALCAM ELISA Kit

规格/价格

96T/2480

指标别名

ALCAM; CD166; CD166 antigen; MEMD

免疫原

NSO, W28-A526

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

312 pg/ml - 20000 pg/ml

基因名称

ALCAM

基因名全称

activated leukocyte cell adhesion molecule

Uniprot ID

Q13740

文献引用格式

Human ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0995)

背景资料

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0995-CAP	96-well plate precoated with anti- human ALCAM antibody	1
EK0995-ST	Lyophilized recombinant human ALCAM standard	20ng/tube×2
EK0995-DA	Biotinylated anti- human ALCAM antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	648	1967	8283	688	2156	8924
Standard deviation	37.58	145.55	563.24	48.16	198.35	651.45
CV(%)	5.8	7.4	6.8	7	9.2	7.3

典型数据示例

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.033	0.109	0.137	0.215	0.385	0.688	1.138	2.063

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human ALCAM ELISA Kit

EK0995

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

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检测方法

样本类型

实验时长

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