Mouse ALCAM ELISA Kit

说明书

EK1184

96T ￥2480
货期：现货

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Mouse ALCAM ELISA Kit standard curve
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产品简介>

产品名称

Mouse ALCAM ELISA Kit

规格/价格

96T/2480

指标别名

ALCAM; CD166; CD166 antigen; MEMD

免疫原

Expression system for standard: NS0; Immunogen sequence: W28-K527

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

敏感性

<10pg/ml

检测范围

78pg/ml-5000pg/ml

基因名称

Alcam

基因名全称

CD166 antigen

Uniprot ID

Q61490

文献引用格式

Mouse ALCAM ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1184)

背景资料

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

试剂盒内容

Catalog number	Description	Quantity
EK1184-CAP	96-well plate precoated with anti- mouse ALCAM antibody	1
EK1184-ST	Lyophilized recombinant mouse ALCAM standard	10ng/tube×2
EK1184-DA	Biotinylated anti- mouse ALCAM antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	116	727	2784	112	706	2677
Standard deviation	8.58	29.8	219.93	8.28	32.47	211.48
CV(%)	7.4%	4.1%	7.9%	7.4%	4.6%	7.9%

典型数据示例

Concentration(pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.066	0.098	0.164	0.294	0.544	1.007	1.821	2.388

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse ALCAM ELISA Kit

EK1184

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

敏感性

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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