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Human Granzyme B/GZMB ELISA Kit

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EK1114

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human Granzyme B/GZMB ELISA Kit
规格/价格
96T/2480
指标别名
CCPI; CGL 1; CGL1; CSP B; CSPB; CTLA1; CTSGL1; Endogenous granzyme B; Granzyme B; GZMB; HLP; SECT
免疫原
NSO, I21-Y247
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
敏感性
<10 pg/ml
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
GZMB
基因名全称
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Uniprot ID
P10144  
文献引用格式
Human Granzyme B/GZMB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1114)
背景资料
Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes(CTL) and natural killer(NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1114-CAP96-well plate precoated with anti- human Granzyme B antibody1
EK1114-STLyophilized recombinant human Granzyme B standard10ng/tube×2
EK1114-DABiotinylated anti- human Granzyme B antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3517047638176487
Standard deviation2.737.1427.63.2610.232.62
CV(%)7.84.25.88.65.86.7
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0450.1190.1760.3380.5901.0191.6632.424
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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